|
KMID : 0368420080510010058
|
|
Journal of Plant Biology
2008 Volume.51 No. 1 p.58 ~ p.63
|
|
|
Astaxanthin production in transgenicArabidopsis with chyB gene encoding β;-carotene Hydroxylase
|
|
Cho Deog-Hee
Jung Yu-Jin
Choi Chang-Sun
Lee Ho-Jae
Pairk Jin-Heui
Dane Fenny
Kang Kwon-Kyoo
|
|
|
Abstract
|
|
|
|
Oxycarotenoids, produced through the oxidation of carotenoids, play critical roles in plants. This reaction is mediated by a specific enzyme, β;-carotene hydroxylase, which adds hydroxyl groups to the β;-rings of carotenes. To investigate the effect of the β;-carotene hydroxylase gene (Chyb) on oxycarotenoid biosynthesis, we generated transgenicArabidopsis plants that over-expressedChyb under the control of a 35S promoter. Their levels of zeaxanthin and neoxanthin were two- to three-fold greater relative to the WT, while that of violaxanthin, a final product in the xanlthophyll pathway, was 1.3-fold higher than the control. In contrast, the amount of β;-carotene declined as much as 2.4-fold, depending on the particular transgenic line. Interestingly, astaxanthin was produced in the transgenics, but not in the WT. These data suggest that, with the aid of unknown factors in the host, carotenoids could be converted into metabolites in the astaxanthin biosynthetic pathway. Microarray analysis was used lo identify several genes that were consistently up-or down-regulated in transgenic chyB leaves compared with the controls. Here, we also discuss possible modifications in leaf carotenoids, and the importance of these data from a nutritional standpoint.
|
|
|
KEYWORD
|
|
|
Arabidopsis, astaxanthin, ß-carotene hydroxylase, microarray, oxycarotenoids, transgenic plant
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|